Regulated gene insertion by steroid-induced C31 integrase
نویسندگان
چکیده
منابع مشابه
Regulated gene insertion by steroid-induced ΦC31 integrase
Nonviral integration systems are widely used genetic tools in transgenesis and play increasingly important roles in strategies for therapeutic gene transfer. Methods to efficiently regulate the activity of transposases and site-specific recombinases have important implications for their spatiotemporal regulation in live transgenic animals as well as for studies of their applicability as safe ve...
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Few nonviral techniques exist for efficient and stable eukaryotic gene transfer and fewer still are broadly useful in both cell culture and whole-organism applications. C31 integrase, a site-specific bacteriophage recombinase, is able to catalyze chromosomal transgene insertion under a diverse range of experimental and therapeutic conditions. The enzyme recognizes and catalyzes unidirectional r...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkn298